Complementary Target Engagement and Functional Assays to Probe NLRP3 Pathway Antagonism

Time: 10:00 am
day: Day One


  • Three high throughput amenable, cell-based methods to support inflammasome drug discovery
  • NLRP3 inhibitors verified with multiplexed caspase-1 activity assay and IL-1β immunoassay
  • Live-cell NLRP3 BRET target engagement assay used to probe several NLRP3 inhibitors
  • Establish a relationship between the target binding and functional effects of NACHT domain inhibitors in live cells